Abstract Detail

Abstract

Dentin and bone consist of 70% apatite, 20% collagen and 10% body fluid in weight. C.B.Huggins (Nobel award winner) and M.R.Urist (BMP discoverer) reported in 1970 that rat demineralized dentin matrix (DDM) induced bone at 4 weeks1). We confirmed human DDM induced bone and cartilage independently at 4 weeks. The demineralization process for dentin and bone increases their osteo-inductivity and kills bacteria. Several shapes of DDM can be prepared. We achieved the DDM autograft for sinus augmentation as a first clinical case in 2002 for dental implants, and published the 20 years-follow up2). Our idea is a bio-recycle use of the extracted tooth as an absorbable, collagenous material with natural cross-linking and growth factors for bone regeneration3). Case 1: A 46-year-old male presented a sever atrophic posterior left maxilla. Autograft using non-functional teeth (#17,18)-derived partially demineralized dentin matrix (DDM) granules was done for sinus augmentation in 2002. Bone biopsies at 5 months were carried out from the implant sites, and three fixtures were placed into the augmented bone. The biopsy tissues showed continuous trabecular bone connected with DDM. Newly formed bone was found on DDM and the surface of DDM appeared slightly irregular. Dental X-ray photos in 2009 and 2021 showed the healthy bone shadow near the fixture-necks, and the top outline of the augmented bone showed irregular. Case 2: A 66-year-old female presented a bony healing failure in the extracted socket (#36) for 2 months. A non-functional, vital wisdom tooth was crushed by our newly developed electric mill, decalcified in 2.0% HNO3 solution, and rinsed extensively in cold distilled water. The immediate graft of DDM was carried out in 2009, and biopsy was taken at 5 months after surgery. The biopsy tissue showed that new bone formation occurred directly on dentin and cementum area. DDM was harmonized with the original tissue, and remodeled by bone. These evidences indicated that DDM could be recycled for successful bone regeneration, and opened a novel way as biological scaffolds. In this seminar, Dentin Science will be introduced for patients in wonderful Paris.Dentin matrix transformation. Science 167:896-898, 1970 Twenty Years-Passed Case of Demineralized Dentin Matrix Autograft for Sinus Bone Augmentation - A First Case of Dentin Graft in Human –. Journal of Clinical and Experimental Dentistry, 15(10) e861-5, 2023 Advances in Oral Tissue Engineering. Masaru Murata, In-Woong Um (Ed.), Quintessence Publishing, 2014

What will the audience take away from your presentation?

Dentin autograft with histological evidences
Dentin matrix includes growth factors as similar to bone
How to prepare DDM in dental clinic
Successful DDM cases in Japan and Korea

Biography

1988 D.D.S., Schoolof Dentistry, HokkaidoUniversity, Sapporo, Japan 1988 Residency, Oral Surgery, Hokkaido University

1993 Ph.D., Postgraduate School of Dentistry, Hokkaido University 1993 Assistant Professor, Oral Pathology, Okayama University

1995 Researcher, Medical Biology, University of Louis Pasteur,France

1997 Assistant Professor, Oral and Maxillofacial Surgery, Health SciencesUniversity of Hokkaido 1999 Lecturer, Health Sciences University of Hokkaido (HSUH)

2004-5 Project leader,Bio-recycle Medical System of Teeth, Japan METI grant 2007 Associate Professor, Oral and Maxillofacial Surgery, HSUH, Japan

2013-6 Visiting Professor, Pioneer Dental College,Dhaka, Bangladesh (concurrent) 2017 Chief Professor, Oral Regenerative Surgery, HSUH, Japan

Patents: Japanese patent: 3, Oversea:1 (USA)